This special issue of Rice covers the use of genome editing technology, its use to better understand rice plants, and how applications of the technology can improve crop yield and increase quality. Masaki Endo and Seiichi Toki are Guest Editors for the series.
Genome Editing
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Citation: Rice 2020 13:27
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An In Vivo Targeted Deletion of the Calmodulin-Binding Domain from Rice Glutamate Decarboxylase 3 (OsGAD3) Increases γ-Aminobutyric Acid Content in Grains
Gamma-aminobutyric acid (GABA) is a non-protein amino acid present in all living things. GABA is mainly synthesized from glutamate by glutamate decarboxylase (GAD). In plants the enzymatic activity of GAD is a...
Citation: Rice 2020 13:20 -
How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis
The breakthrough CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated genome-editing technology has led to great progress in monocot research; however, several factors need to be con...
Citation: Rice 2020 13:9 -
Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make...
Citation: Rice 2020 13:8 -
Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene
Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes. Another technical advantage is that RNP delivery can...
Citation: Rice 2020 13:4 -
Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants
Continuing crop domestication/redomestication and modification is a key determinant of the adaptation and fulfillment of the food requirements of an exploding global population under increasingly challenging c...
Citation: Rice 2019 12:95 -
A novel approach to carotenoid accumulation in rice callus by mimicking the cauliflower Orange mutation via genome editing
β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed...
Citation: Rice 2019 12:81