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Genome Editing

This special issue of Rice covers the use of genome editing technology, its use to better understand rice plants, and how applications of the technology can improve crop yield and increase quality. Masaki Endo and Seiichi Toki are Guest Editors for the series.

  1. Gamma-aminobutyric acid (GABA) is a non-protein amino acid present in all living things. GABA is mainly synthesized from glutamate by glutamate decarboxylase (GAD). In plants the enzymatic activity of GAD is a...

    Authors: Kazuhito Akama, Nadia Akter, Hinako Endo, Masako Kanesaki, Masaki Endo and Seiichi Toki
    Citation: Rice 2020 13:20
  2. The breakthrough CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated genome-editing technology has led to great progress in monocot research; however, several factors need to be con...

    Authors: Jin-Jun Yue, Chwan-Yang Hong, Pengcheng Wei, Yu-Chang Tsai and Choun-Sea Lin
    Citation: Rice 2020 13:9
  3. Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make...

    Authors: Zhaohui Zhong, Shishi Liu, Xiaopei Liu, Binglin Liu, Xu Tang, Qiurong Ren, Jianping Zhou, Xuelian Zheng, Yiping Qi and Yong Zhang
    Citation: Rice 2020 13:8
  4. Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes. Another technical advantage is that RNP delivery can...

    Authors: Raviraj Banakar, Mollie Schubert, Michael Collingwood, Christopher Vakulskas, Alan L. Eggenberger and Kan Wang
    Citation: Rice 2020 13:4
  5. β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed...

    Authors: Akira Endo, Hiroaki Saika, Miho Takemura, Norihiko Misawa and Seiichi Toki
    Citation: Rice 2019 12:81